Lipopolysaccharide(LPS)from activates the Toll-like receptor 4(TLR4)signaling pathway,inducing acute inflammation,sepsis......

Lipopolysaccharide(LPS)fromactivates the Toll-like receptor 4(TLR4)signaling pathway,inducing acute inflammation,sepsis,......

目的:构建、表达、纯化抗血管内皮生长因子(VEGF)的Fab片段(眼明,Vasculizumab),并检测其抗VEGF的活性.方法:首先对轻链(LC)及重链......

将抗ＨＢｓＡｇ抗体的轻链和重链Ｆｄ段基因，分别克隆于ｐＥＴ２０ｂ质粒中并分别转化到大肠肝菌ＢＬ２１（ＤＥ３），ＬＰＴＧ诱导后，ＳＤＳ－ＰＡＧＥ分析发现在２７ＫＤ（Ｌ）和２５ＫＤ（Ｆｄ）处３有外源蛋白表达，表达蛋白含量分别为５３％和４８。Ｌ链......